MKK6/MEK6 [p Ser207] Antibody

Western Blot: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) antibody

Immunohistochemistry-Paraffin: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82050(left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/Immunofluorescence: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Staining of methanol-fixed Hela cells showing cytoplasmic staining using NB100-82050

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MKK6/MEK6 [p Ser207] Antibody Summary

• Description: Novus Biologicals Rabbit MKK6/MEK6 [p Ser207] Antibody (NB100-82050) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MKK6/MEK6 around the phosphorylation site of serine 207 (V-D-SP-V-A).
• Modification: p Ser207
• Specificity: Detects endogenous levels of MKK6/MEK6 only when phosphorylated at serine207.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP2K6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MKK6/MEK6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKK6/MEK6 [p Ser207] Antibody

• MAP kinase kinase 6
• MAP2K6
• MAPK/ERK kinase 6
• MAPKK6dual specificity mitogen-activated protein kinase kinase 6
• MEK6
• MEK6MAPKK 6
• mitogen-activated protein kinase kinase 6
• MKK6
• MKK6MEK 6
• PRKMK6
• PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
• SAPKK3
• SAPKK3EC 2.7.12.2

Background

Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K6
• Entrez:
  • 5608(Human)
  • 114495(Rat)
• Uniprot:
  • P52564()