MKK6/MEK6 [p Ser207] Antibody

Images

 
Western Blot: MEK6 [p Ser207] Antibody [NB100-82050] - Analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) antibody
Immunocytochemistry/ Immunofluorescence: MEK6 [p Ser207] Antibody [NB100-82050] - Staining of methanol-fixed Hela cells showing cytoplasmic staining using MKK6(Phospho-Ser207) antibody
Immunohistochemistry: MEK6 [p Ser207] Antibody [NB100-82050] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using MKK6 (phospho-Ser207) antibody
Western Blot: MEK6 [p Ser207] Antibody [NB100-82050] - Western blot analysis of extract from MDA-MB-435 cells untreated or treated with UV, using MKK6 (Ab-207) antibody (Lane 1 and 2) and MKK6 (phospho-Ser207) antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MKK6/MEK6 [p Ser207] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MKK6 around the phosphorylation site of serine 207 (V-D-SP-V-A).
Modification
p Ser207
Specificity
MKK6 (specific to Phospho-Ser207) detects endogenous levels of MKK6 only when phosphorylated at serine207.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MKK6/MEK6 Lysate (NBL1-12851)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS [pH 7.4] contains 150mM NaCl and 50% glycerol (without Mg2+ and Ca2+)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKK6/MEK6 [p Ser207] Antibody

  • MAP kinase kinase 6
  • MAP2K6
  • MAPK/ERK kinase 6
  • MAPKK6dual specificity mitogen-activated protein kinase kinase 6
  • MEK6
  • MEK6MAPKK 6
  • mitogen-activated protein kinase kinase 6
  • MKK6
  • MKK6MEK 6
  • PRKMK6
  • PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
  • SAPKK3
  • SAPKK3EC 2.7.12.2

Background

Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP2K6
Entrez
Uniprot