MKK6/MEK6 Overexpression Lysate

Images

 
Western Blot: MEK6 Overexpression Lysate (Adult Normal) [NBL1-12851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MKK6/MEK6 Overexpression Lysate Summary

Description
Transient overexpression lysate of mitogen-activated protein kinase kinase 6 (MAP2K6) The lysate was created in HEK293T cells, using Plasmid ID RC204481 and based on accession number NM_002758. The protein contains a C-MYC/DDK Tag.
Gene
MAP2K6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MKK6/MEK6 Overexpression Lysate

  • MAP kinase kinase 6
  • MAP2K6
  • MAPK/ERK kinase 6
  • MAPKK6dual specificity mitogen-activated protein kinase kinase 6
  • MEK6
  • MEK6MAPKK 6
  • mitogen-activated protein kinase kinase 6
  • MKK6
  • MKK6MEK 6
  • PRKMK6
  • PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
  • SAPKK3
  • SAPKK3EC 2.7.12.2

Background

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MKK6/MEK6 Lysate (NBL1-12851) (0)

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NBL1-12851

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Bioinformatics

Gene Symbol MAP2K6