MKK4/MEK4 Antibodies
We offer MKK4/MEK4 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each MKK4/MEK4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MKK4/MEK4 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the MKK4/MEK4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MKK4/MEK4 polyclonal antibodies and browse our MKK4/MEK4 monoclonal antibody catalog.
Alternate Names for MKK4/MEK4 Antibodies
anti-MKK4/MEK4 antibody, anti-MAP2K4 antibody, anti-c-Jun N-terminal kinase kinase 1 antibody, anti-dual specificity mitogen-activated protein kinase kinase 4 antibody, anti-JNK activating kinase 1 antibody, anti-JNK-activated kinase 1 antibody, anti-JNK-activating kinase 1 antibody, anti-JNKK antibody, anti-JNKK1MAPK/ERK kinase 4 antibody, anti-MAP kinase kinase 4 antibody, anti-MAPKK4 antibody, anti-MEK4MEK 4 antibody, anti-mitogen-activated protein kinase kinase 4 antibody, anti-MKK4SAPK/ERK kinase 1 antibody, anti-PRKMK4MAPKK 4 antibody, anti-SEK1 antibody, anti-SERK1EC 2.7.12.2 antibody
19 Results for "mkk4-mek4" in Primary Antibodies
201-LB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-MKK4 (S257/T261) at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 300 mM sorbitol for 30 minutes and HepG2 human hepatocellular carcinoma cell line untreated or treated with 10 ng/mL Recombinant Human IL-1 beta (201-LB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MKK4 (S257/T261) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2990), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-MKK4 (S257/T261) at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="MKK4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human MKK4 Monoclonal Antibody (Catalog # MAB3390) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Western-Blot-NBP1-47839-img0020.jpg "Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0013.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0022.jpg)
![Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Flow-Cytometry-NBP1-47839-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunocytochemistry-Immunofluorescence-NBP1-47839-img0012.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0014.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0015.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0017.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunohistochemistry-Paraffin-NBP1-47839-img0018.jpg)
![Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-OTI8A8-Immunoprecipitation-NBP1-47839-img0021.jpg)
![Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Western-Blot-NBP3-03652-img0002.jpg "Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![<b>Genetic Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Knockout-Validated-NBP3-03652-img0001.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251636328.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516345226.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of lysates from A-549 cells, using MKK4/MEK4 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516293950.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251633412.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Rat lung using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-5220251640210.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of various lysates, using MKK4/MEK4 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516283264.jpg)
![Immunohistochemistry: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MKK4/MEK4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516283227.jpg)
![Western Blot: MKK4/MEK4 Antibody - BSA Free [MKK4/MEK4] - Western blot analysis of lysates from HeLa cells, using MKK4/MEK4 Rabbit pAb at 1:700 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03652_rabbit-polyclonal-mkk4-mek4-antibody-52202516345299.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249355552.jpg "Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024940882.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-212024938311.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249413375.jpg)
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - SEK1 / MKK4 antibody [HL1839] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249362735.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1839) - Azide and BSA Free [NBP3-25674] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (NBP3-25674) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25674_rabbit-mkk4-mek4-mab-hl1839-azide-and-bsa-free-2120249403888.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249432880.jpg "Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249403813.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Untransfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249415916.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-212024941452.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249355531.jpg)
![Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25675_rabbit-mkk4-mek4-mab-hl1888-azide-and-bsa-free-2120249422682.jpg)
NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for ![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0003.jpg "Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunohistochemistry-Paraffin-NBP2-99331-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99331 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99331_rabbit-polyclonal-mkk4-mek4-antibody-23520252145179.png)
![Western Blot: MKK4/MEK4 Antibody [NBP2-57194] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Western-Blot-NBP2-57194-img0002.jpg "Western Blot: MKK4/MEK4 Antibody [NBP2-57194] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-57194] - Staining of human cell line HEK 293 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57194-img0001.jpg)
![Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of 50ug whole cell lysate.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-2-E4-Western-Blot-NBP2-22482-img0001.jpg "Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of 50ug whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of MAP2K4 using anti-MAP2K4 monoclonal antibody shows specific expression in HeLa cells (shown in green) but not in negative control NRK cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing MAP2K4, at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-554 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/MKK4-MEK4-Antibody-2-E4-Immunocytochemistry-Immunofluorescence-NBP2-22482-img0003.jpg)
