Mitofusin 2 Antibody

Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-17298] - Paraffin-embedded mouse fore brain. MFN2 antibody [N1N2], N-term dilution: 1:500.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MFN2 antibody [N1N2], N-term (NBP2-17298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Mitofusin 2 Antibody Summary

• Description: Novus Biologicals Rabbit Mitofusin 2 Antibody (NBP2-17298) is a polyclonal antibody validated for use in IHC and WB. Anti-Mitofusin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Mitofusin 2. The exact sequence is proprietary.
• Predicted Species: Rat (96%), Canine (98%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MFN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%), Zebrafish (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Mitofusin 2 Antibody

• CMT2A
• CMT2A2
• CPRP1
• CPRP1mitochondrial assembly regulatory factor
• EC 3.6.5
• EC 3.6.5.-
• HSG
• KIAA0214hyperplasia suppressor
• MARF
• MFN2
• Mitofusin 2
• mitofusin-2
• Transmembrane GTPase MFN2

Background

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Mitofusin 2 Antibody (NBP2-17298)(1)

Publication using NBP2-17298

• Jingwen Zhang, Zhibin He, Julia Fedorova, Cole Logan, Lauryn Bates, Kayla Davitt, Van Le, Jiayuan Murphy, Melissa Li, Mingyi Wang, Edward G. Lakatta, Di Ren, Ji Li Alterations in mitochondrial dynamics with age‐related Sirtuin1/Sirtuin3 deficiency impair cardiomyocyte contractility Aging Cell 2021-07-03 [PMID: 34216536]

Bioinformatics

• Gene Symbol: MFN2
• Uniprot:
  • O95140()