Mitofusin 1 Antibody

Western blot shows mitochondrial extracts of HeLa human cervical epithelial carcinoma cell line (20 µg/lane) and Raji human Burkitt's lymphoma cell line (2.5 µg/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Mitofusin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7880) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mitofusin 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Mitofusin 1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Mitofusin 1
  Ala2-Lys77
  Accession # Q8IWA4
• Specificity: Detects human Mitofusin 1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human Mitofusin 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MFN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mitofusin 1 Antibody

• DKFZp762F247
• EC 3.6.5
• EC 3.6.5.-
• FLJ20693
• Fzo homolog
• hfzo1
• hfzo2
• MFN1
• MGC41806
• mitochondrial transmembrane GTPase Fzo-1
• mitochondrial transmembrane GTPase FZO-2
• Mitofusin 1
• mitofusin-1
• putative transmembrane GTPase
• Transmembrane GTPase MFN1

Background

MFN-1 (Mitofusin 1; also Fzo/"fuzzy onions" homolog and Transmembrane GTPase MFN-1) is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. MFN-1 apparently mediates the fusion of individual mitochondria with each other through trans-interactions with adjacent mitochondrial MFN-1 and/or MFN-2. Pertubations with this process result in both a general overall fragmentation of mitochondria, and in cell-specific effects such as a reduction in oxidative phosphorylation or an imbalance in the generation of ROS. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1:MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Human MFN-1 is a 741 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C-terminus. The cytoplasmic N-terminus (aa 1-584) contains the GTPase domain (aa 82-286) while the cytoplasmic C-terminus possesses a critical coiled-coil motif (aa 679-734). MFN-1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. Multiple splice variants are reported to occur. One is cytosolic and contains a four aa substitution for aa 367-741. Three others show a deletion of aa 444-554, aa 326-408 and an alternative start site at Met387, respectively. Over aa 1-77, human and mouse MFN-1 share 88% aa sequence identity. Full-length human MFN-1 and MFN-2 share 61% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitofusin 1 Antibody (AF7880)(3)

Publications using AF7880

• Christopher L. Axelrod, Ciaran E. Fealy, Melissa L. Erickson, Gangarao Davuluri, Hisashi Fujioka, Wagner S. Dantas et al. Lipids activate skeletal muscle mitochondrial fission and quality control networks to induce insulin resistance in humans Metabolism 8/1/2021 [PMID: 34090870]
• Axelrod CL, Fealy CE, Mulya A, Kirwan JP. Exercise Training Remodels Human Skeletal Muscle Mitochondrial Fission and Fusion Machinery Towards a Pro-Elongation Phenotype. Acta Physiol (Oxf). 11/8/2018 [PMID: 30408342]
• J Sacks, A Mulya, CE Fealy, H Huang, JD Mosinski, MR Pagadala, H Shimizu, E Batayyah, PR Schauer, SA Brethauer, JP Kirwan Effect of Roux-en-Y gastric bypass on liver mitochondrial dynamics in a rat model of obesity Physiol Rep, 2018-02-01;6(4):. 2018-02-01 [PMID: 29464885] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: MFN1
• Uniprot:
  • Q8IWA4()