MITF Antibody (MITF/915)

Images

 
Immunohistochemistry-Paraffin: MITF Antibody (MITF/915) [NBP2-45161] - Human Melanoma stained with MITF Monoclonal Antibody (MITF/915).
Immunohistochemistry-Paraffin: MITF Antibody (MITF/915) [NBP2-45161] - Formalin-fixed, paraffin-embedded human Melanoma stained with MITF Antibody (MITF/915).

Product Details

Summary
Reactivity Hu, Ca, Mu(-), Rt(-)Species Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
MITF/915
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

MITF Antibody (MITF/915) Summary

Immunogen
Recombinant full-length human MiTF protein
Localization
Nuclear
Specificity
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MITF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 3p14.1
Molecular weight of antigen: 52-56kDa (doublet)
Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-45161 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MITF Antibody (MITF/915)

  • bHLHe32
  • bHLHe32MI
  • Class E basic helix-loop-helix protein 32
  • MI
  • microphthalmia-associated transcription factor
  • MITF
  • Waardenburg syndrome, type 2A
  • WS2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, IHC, IHC-P, ICC/IF

Publications for MITF Antibody (NBP2-45161) (0)

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Review for MITF Antibody (NBP2-45161) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-45161:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry MITF NBP2-45161
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reviewed by:
Fabio Marongiu
ICC Human 08/29/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedARPE-19 cells
SpeciesHuman
Lot4286-2P160209

Comments

CommentsFixative 4% PFA + 0.1% Triton
Blocking Goat Serum
Dilution 1:200
Incubation 1h @RT

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Recent Reviews

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Fabio Marongiu
08/29/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol MITF