Mind Bomb 1/MIB1 Antibody [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Mind Bomb 1/MIB1 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1. The immunogen is located within amino acids 920 - 970 of MIB1. Amino Acid Squence: IPVLQKDKDNTNVNADVQK
• Specificity: Mind Bomb 1/MIB1 antibody is human, mouse and rat reactive. At least three isoforms of Mind Bomb 1/MIB1 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MIB1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Mind Bomb 1/MIB1 Antibody [CoraFluor™ 1]

• DAPK-interacting protein 1
• DIP1
• DIP-1FLJ90676
• DKFZp686I0769
• DKFZp761M1710
• E3 ubiquitin-protein ligase MIB1
• EC 6.3.2
• EC 6.3.2.-
• KIAA1323
• MGC129660
• MIB
• MIB1
• Mind Bomb 1
• Mind bomb homolog 1
• mindbomb homolog 1 (Drosophila)
• ubiquitin ligase mind bomb
• ubiquitin ligase protein MIB1
• Zinc finger ZZ type with ankyrin repeat domain protein 2
• ZZANK2
• ZZANK2MGC129659
• ZZZ6

Background

E3 ubiquitin ligase protein mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MIB1