MINA Antibody - BSA Free

Western Blot: MINA Antibody [NBP1-89863] - Analysis in control (vector only transfected HEK293T lysate) and MINA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: MINA Antibody [NBP1-89863] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.

Immunohistochemistry-Paraffin: MINA Antibody [NBP1-89863] - Staining of human thyroid gland shows nuclear positivity in glandular cells.

Western Blot: MINA Antibody [NBP1-89863] - Analysis in human cell lines A-431 and HeLa using anti-MINA antibody. Corresponding MINA RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MINA Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MPKKAKPTGSGKEEGPAPCKQMKLEAAGGPSALNFDSPSSLFESLISPIKTETFFKEFWEQKPLLIQRDDPALATYYGSLFKLTDLKSLCSRGMYYGRDVNVCRCVNGKKKVLNKDGKAHFLQLRKDFDQKRATIQFHQPQRFKDELW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIOX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MINA Protein (NBP1-89863PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MINA Antibody - BSA Free

• DKFZp762O1912
• FLJ14393
• MDIG
• MDIGDKFZp762O1912
• MINA
• Mina53
• MINA53mdig
• mineral dust induced gene protein
• Mineral dust-induced gene protein
• MYC induced nuclear antigen
• MYC-induced nuclear antigen
• myc-induced nuclear antigen, 53 kDa
• NO52
• Nucleolar protein 52

Background

MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIOX2