MID1IP1 Antibody - Azide and BSA Free

Western Blot: MID1IP1 Antibody [NBP3-00003] - Anti-MID1IP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 24 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: MID1IP1 Antibody [NBP3-00003] - MID1IP1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-MID1IP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MID1IP1 rabbit polyclonal antibody, at 1:250 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MID1IP1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human MID1IP1 (Uniprot#: Q9NPA3; Met1-His183)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MID1IP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MID1IP1 Antibody - Azide and BSA Free

• FLJ10386
• G12-like
• Gastrulation-specific G12-like protein
• MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))
• MID1 interacting protein 1 (gastrulation specific G12-like (zebrafish))
• MID1 interacting protein 1 (gastrulation specific G12-like)
• Mid1-interacting G12-like protein
• mid1-interacting protein 1
• MIG12MID1 interacting G12-like protein
• Protein STRAIT11499
• S14R
• Spot 14-R
• Spot 14-related protein
• STRAIT11499
• THRSPL

Background

MID1IP1 is involved in stabilization of microtubules

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MID1IP1