MHC Class I Antibody

Western Blot: MHC Class I Antibody [H00003133-D01P] - Analysis of HLA-E transfected lysate using anti-HLA-E rabbit polyclonal antibody and Protein Magnetic Bead, and immunoblotted with HLA-E purified mouse polyclonal antibody (B01P).

Western Blot: MHC Class I Antibody [H00003133-D01P] - Analysis of HLA-E expression in transfected 293T cell line by HLA-E polyclonal antibody.Lane 1: HLA-E transfected lysate(40.20 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

MHC Class I Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: HLA-E (AAH02578.1, 1 a.a. - 358 a.a.) full-length human protein. MVDGTLLLLLSEALALTQTWAGSHSLKYFHTSVSRPGRGEPRFISVGYVDDTQFVRFDNDAASPRMVPRAPWMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGYYNQSEAGSHTLQWMHGCELGPDRRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQQDGEGHTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPVTLRWKPASQPTIPIVGIIAGLVLLGSVVSGAVVAAVIWRKKSSGGKGGSYSKAEWSDSAQGSESHSL
• Specificity: HLA-E,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLA-E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MHC Class I Antibody

• DKFZp686P19218
• EA1.2
• EA2.1
• HLA class I histocompatibility antigen, alpha chain E
• HLA class I histocompatibility antigen, E alpha chain
• HLA-6.2
• HLAE
• lymphocyte antigen
• major histocompatibility complex, class I, E
• MHC class I antigen E
• MHC HLA-E alpha-1
• MHC HLA-E alpha-2.1
• MHC
• QA1

Background

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-E
• Entrez:
  • 3133(Human)
• Uniprot:
  • AAH02578.1(Human)