NB120-15680 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k,q,s. Mouse strains with the haplotypes H-2p,r,w7,w22 show weak reactivity with this antibody.
Protein A or G purified
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IHC-P: Does not react on routinely fixed paraffin sections (4% formalin). Use at a fixation of 1% formalin or glutaraldehyde). Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Read Publication using NB120-15680 in the following applications:
MHC Class I and the Herpes Simplex Virus MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens. These molecules are highly polymorphic, meaning that they exist in multiple varian... Read full blog post.
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