MHC Class I Antibody (ERMP42)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
ERMP42
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MHC Class I Antibody (ERMP42) Summary

Immunogen
Balb/c macrophage precursor cell hybrids.
Specificity
NB120-15680 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k,q,s. Mouse strains with the haplotypes H-2p,r,w7,w22 show weak reactivity with this antibody.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
HLA-E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
IHC-P: Does not react on routinely fixed paraffin sections (4% formalin). Use at a fixation of 1% formalin or glutaraldehyde). Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Publications
Read Publication using
NB120-15680 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MHC Class I Antibody (ERMP42)

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class I Antibody (NB120-15680)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
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(1)
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Secondary Antibodies

 

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Blogs on MHC Class I.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-E