MHC Class I Antibody (ER-HR52) - BSA Free

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
ER-HR52
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

MHC Class I Antibody (ER-HR52) - BSA Free Summary

Immunogen
Adherent FI (CBAXBL) bone marrow stromal cells
Localization
Cell Membrane; Type I membrane protein.
Specificity
NB100-64952 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP52 specifically recognizes mouse strains with the haplotypes H-2b,w16 and H-2d,p,q. Mouse strains with the haplotypes H-2f,r,s,w17,w23,w27 show weak reactivity with this antibody.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
HLA-E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 29195074).
Publications
Read Publications using
NB100-64952 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MHC Class I Antibody (ER-HR52) - BSA Free

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class I Antibody (NB100-64952)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(1)
IHC
(1)
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Showing Publications 1 - 2 of 2.
Publications using NB100-64952 Applications Species
Kortlever Roderik M, Sodir Nicole M, Wilson Catherine H et al. Myc CoopeRates with Ras by Programming Inflammation and Immune Suppression. Cell 2017 [PMID: 29195074] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Torrente D, Su E, Schielke G et al. Tissue Plasminogen Activator Interaction with NMDAR1 Promotes Dopaminergic Neuron Degeneration in a Model of A-Synuclein-Mediated Neurotoxicity SSRN Electronic Journal Mar 22 2022

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class I Antibody (NB100-64952) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on MHC Class I.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-E
Entrez