mGluR5 Antibody (464818) [Unconjugated]

MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB4514, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 464818
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

mGluR5 Antibody (464818) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human mGluR5
  Gln21-Ser509
  Accession # P41594
• Specificity: Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human mGluR1, R2, R3, R4, R7, or R8 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRM5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for mGluR5 Antibody (464818) [Unconjugated]

• glutamate receptor, metabotropic 5
• GPRC1E
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• GRM5
• mGlu5
• mGluR5

Background

Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 aa in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca⁺⁺-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat, and dog mGluR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for mGluR5 Antibody (MAB4514) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 02/20/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 24236200
• Species: Human

Bioinformatics

• Gene Symbol: GRM5
• Uniprot:
  • P41594()