mGluR5 Antibody (464818) [Unconjugated]

Images

 
MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human mGluR5 Monoclonal Antibody (Catalog # MAB4514, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
464818
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

mGluR5 Antibody (464818) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR5
Gln21-Ser509
Accession # P41594
Specificity
Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human mGluR1, R2, R3, R4, R7, or R8 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GRM5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
MAB4514 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for mGluR5 Antibody (464818) [Unconjugated]

  • glutamate receptor, metabotropic 5
  • GPRC1E
  • GPRC1EMGLUR5metabotropic glutamate receptor 5
  • GRM5
  • mGlu5
  • mGluR5

Background

Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 aa in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat, and dog mGluR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for mGluR5 Antibody (MAB4514) (0)

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Review for mGluR5 Antibody (MAB4514) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4514:
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Flow
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 02/20/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24236200
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24236200

Details

Detection NotesSee PMID 24236200

Comments

CommentsIndependent review from antibodyresource.com

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Recent Reviews

4
5
0
4
1
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0
2
0
1
0

 
Anonymous
02/20/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol GRM5
Uniprot