mGluR2/3 Antibody

Western Blot: metabotropic Glutamate Receptor 2/3 Antibody [NBP2-21064] - Rat brain shows bands migrating at Mr=100,000 and 190,000 which may be a dimer of the smaller band.

Product Discontinued

mGluR2/3 Antibody Summary

• Immunogen: NGREVVDSTTSSL Corresponding to the carboxy-terminus of rat mGLuR2
• Specificity: This antibody recognizes a conserved sequence in both metabotropic glutamate receptor 2 and metabotropic glutamate receptor 3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRM2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:30-1:100
  • Immunohistochemistry 1:30-1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:250-1:500
• Application Notes: Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.

Reactivity Notes

Rat and Mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with Tris-Glycine (pH 7.8) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for mGluR2/3 Antibody

• GLUR2
• glutamate metabotropic receptor 2
• glutamate receptor, metabotropic 2
• GPRC1Bmetabotropic glutamate receptor 2
• mGlu2
• mGluR2
• mGluR2/3
• MGLUR2glutamate receptor homolog

Background

Metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors activated by glutamate. Based on sequence similarity, transduction mechanisms and agonist potencies, mGluRs are subdivided into three groups: mGluR1/mGluR5, mGluR2/mGluR3, and mGluR4/mGluR6/mGluR7/mGluR8. mGluRs are widely distributed throughout the nervous system and are expressed by both neurons and glial cells. mGluRs have been suggested to play a variety of functional roles, among which is involvement in synaptic plasticity underlying learning and memory as well as chronic pain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRM2