MGAT4B Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MGAT4B Antibody [NBP3-17394] - Staining of human cell line RT4 shows localization to nucleoplasm & cytosol.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MGAT4B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MGAT4B Antibody - BSA Free (NBP3-17394) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VVDVYQREFLALRDRLHAAEQESLKRSKELNLVLDEIKRAVSERQALRDGDGNRTWGRLTEDPRLKPWNGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRTGVSVVMGIPSVRREVHSYLTDTLHSLISELSPQEKE
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MGAT4B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MGAT4B Antibody - BSA Free

• alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
• alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• aminyltransferase
• EC 2.4.1.145
• GlcNAc-T IVb
• GNT-IV
• GNT-IVB
• isoenzyme B
• isozyme B
• mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• N-acetylglucosaminyltransferase IVb
• N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
• UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IV
• UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IVb

Background

MGAT4B, also known as Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B, has 3 isoforms, a 548 amino acid isoform that is 63 kDa, a 547 amino acid isoform that is 63 kDa, and a 563 amino acid isoform that is 65 kDa; participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans; acts as a catalyzer in the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans; necessary for the production of tri- and tetra-antennary N-linked sugar chains, and it is not the main contributor in N-glycan biosynthesis. Disease research is currently being performed with relation to MGAT4B and immunodeficiency, pancreatic cancer, hepatocellular carcinoma, pancreatitis, interferon, carcinoma, and extrahepatic bile duct carcinoma. Interactions with this protein have been shown to involve LRSAM1, MGAT2, MGAT3, MGAT5, FUT8, and MGAT5B in N-glycan antennae elongation in the medial/trans-Golgi, transport to the Golgi and subsequent modification, asparagine N-linked glycosylation, N-Glycan antennae elongation, methabolism of proteins, N-Glycan biosynthesis, and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MGAT4B