MGAT4A Antibody (4H4) - Azide and BSA Free

Western Blot: MGAT4A Antibody (4H4) [H00011320-M02] - MGAT4A monoclonal antibody (M02), clone 4H4 Analysis of MGAT4A expression in Jurkat.

Immunohistochemistry-Paraffin: MGAT4A Antibody (4H4) [H00011320-M02] - Analysis of monoclonal antibody to MGAT4A on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.2 ug/ml.

Sandwich ELISA: MGAT4A Antibody (4H4) [H00011320-M02] - Detection limit for recombinant GST tagged MGAT4A is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clone: 4H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MGAT4A Antibody (4H4) - Azide and BSA Free Summary

• Immunogen: MGAT4A (NP_036346, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVNVESYLFHSGNQEHPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIGKFENGVAEGMVDPSLNPISAFRLSVIQNSAVWAILNEIHIKKATN
• Specificity: MGAT4A - mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MGAT4A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested. Rat reactivity reported in scientific literature (PMID: 26104473)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MGAT4A Antibody (4H4) - Azide and BSA Free

• alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
• alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• EC 2.4.1.145
• GlcNAc-T IVa
• GnT-4a
• GNT-IV
• GNT-IVA
• isoenzyme A
• isozyme A
• mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• MGAT4A
• N-Acetylglucosaminyltransferase IVa
• N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
• UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV
• UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IVa
• UDP-N-acetylglucosamine:alpha1,3-d-mannosidebeta1,4-N-acetylglucosaminyltransferase

Background

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MGAT4A Antibody (H00011320-M02)(1)

Publication using H00011320-M02

• Kippe JM, Mueller TM, Haroutunian V, Meador-Woodruff JH. Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia Schizophr. Res. 2015-06-20 [PMID: 26104473] (WB, Human, Rat)

Bioinformatics

• Gene Symbol: MGAT4A
• Entrez:
  • 11320(Human)
• OMIM:
  • 604623(Human)
• Uniprot:
  • NP_036346(Human)