Species: Hu
Applications: IHC
Host: Rabbit Polyclonal
Species: Hu
Applications: Enzyme Activity
Description
Recombinant human Alpha-1,3-mannosyl-glycoprotein 4-beta -N-acetylglucosaminyltransferase A (MGAT4A), also known as GlcNAc-T IVa , is a metal-dependent, golgi single-pass type II membrane protein that contains an N-terminal transmembrane region, a catalytic domain, and a C-terminal carbohydrate binding module that regulates its catalytic activity (1, 2). MGAT4A is one of several human MGAT enzymes involved in the initiation and synthesis of N-glycans, with each enzyme having unique specificity and preferences (3). MGAT4 has two human isozymes that catalyze formation of the beta 1-4 GlcNAc branch in the alpha 1-3 Mannose arm of N‑glycans (4-6). The two isozymes have different expression profiles in tissues and cancer cell lines , with MGAT4A expression within gastrointestinal tissues such as the pancreas (2, 4, 5). Loss of MGAT4A in mouse models has been shown to induce type 2 diabetes (9,10). Expression and activity of MGAT4A is particularly important during differentiation and oncogenesis (7, 8) including within choriocarcinoma, invasive mole, and placental site trophoblastic tumors (11-13). The discovery of MGAT4A modulators may lead to new therapeutics for the treatment of these diseases (10). MGAT4A may also be useful as a tool for the glycoengineering of complex N-glycans. The activity of MGAT4A was demonstrated using a fluorescent gel shift assay.
Bioinformatics
| Uniprot |
Human
|
| Product By Gene ID |
11320 |
| Alternate Names |
- alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
- alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
- EC 2.4.1.145
- GlcNAc-T IVa
- GnT-4a
- GNT-IV
- GNT-IVA
- isoenzyme A
- isozyme A
- mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
- MGAT4A
- N-Acetylglucosaminyltransferase IVa
- N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
- UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV
- UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IVa
- UDP-N-acetylglucosamine:alpha1,3-d-mannosidebeta1,4-N-acetylglucosaminyltransferase
|
![Enzyme Activity MGAT4A [Unconjugated]](http://images.novusbio.com/fullsize/11496-gt_recombinant-human-mgat4a-his-tag-cf-enzyme-activity-532024143843.jpg)
![Enzyme Activity MGAT4A [Unconjugated]](http://images.novusbio.com/fullsize/11496-gt_recombinant-human-mgat4a-his-tag-cf-enzyme-activity-532024144139.jpg)
![SDS-PAGE MGAT4A [Unconjugated]](http://images.novusbio.com/fullsize/11496-gt_recombinant-human-mgat4a-his-tag-cf-sds-page-532024143632..jpg)