Methylmalonyl Coenzyme A mutase Antibody

Western Blot: Methylmalonyl Coenzyme A mutase Antibody [NBP2-97332] - Anti-Methylmalonyl Coenzyme A mutase rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 83 kDa

Immunohistochemistry-Paraffin: Methylmalonyl Coenzyme A mutase Antibody [NBP2-97332] - Immunochemical staining of human Methylmalonyl Coenzyme A mutase in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Methylmalonyl Coenzyme A mutase Antibody [NBP2-97332] - Methylmalonyl Coenzyme A mutase was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. 4 ul anti-Methylmalonyl Coenzyme A mutase rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Methylmalonyl Coenzyme A mutase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 85 kDa

Product Discontinued

Methylmalonyl Coenzyme A mutase Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Methylmalonyl Coenzyme A mutase fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMUT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 100 mM Tris,100 mM Glycine,10 mM NaCL, pH 8.0
• Preservative: 0.03% Proclin
• Purity: Antigen Affinity-purified

Alternate Names for Methylmalonyl Coenzyme A mutase Antibody

• EC 5.4.99.2
• MCMmethylmalonyl Coenzyme A mutase
• methylmalonyl CoA mutase
• Methylmalonyl-CoA isomerase
• methylmalonyl-CoA mutase, mitochondrial

Background

Methylmalonyl Coenzyme A mutase encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMUT