MESDC2 Antibody (513802)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human MESDC2 Monoclonal Antibody (Catalog # MAB5577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MESDC2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 513802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MESDC2 Antibody (513802) Summary

• Immunogen: E. coli-derived recombinant human MESDC2
  Ala34-Leu234
  Accession # Q14696
• Specificity: Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse MESDC2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MESDC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MESDC2 Antibody (513802)

• BOCA
• KIAA0081Renal carcinoma antigen NY-REN-61
• MESDC2
• MESDLDLR chaperone MESD
• mesoderm development candidate 2Mesoderm development protein

Background

Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MESDC2
• Uniprot:
  • Q14696()