Recombinant Human Mer GST Protein

SDS-Page: Recombinant Human Mer Protein [P5596] - 1 ug protein

Product Discontinued

Recombinant Human Mer GST Protein Summary

• Description: A partial recombinant protein with a GST-tag and corresponding to the amino acids 528 - 999 of Human Mer
  
  

  Source: baculovirus infected insect cell (Sf21) expression system

  Amino Acid Sequence: NP_006334

• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Details of Functionality: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CSKtide. ATP: 100 uM.
• Source: Sf 21 (baculovirus)
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: MERTK
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
• Preservative: Glycerol
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Recombinant Human Mer GST Protein

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

MerTK (c-mer, Nyk, Eyk) is a tyrosine kinase that belongs to a family of transmembrane receptors (1). MerTk has been implicated in reversible cell growth arrest, survival, proliferation and cell adhesion. Growth arrest specific protein 6 (Gas6) is an activating ligand for MerTK (2-3). MerTK is required for clearance of apoptotic cells by mononuclear phagocytes in mice; with its absence resulting in progressive lupus like autoimmunity (4). Defects in MERTK are a cause of retinitis pigmentosa (RP) (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MERTK