Menin Antibody [Biotin]

Western Blot: Menin Antibody [Biotin] [NBP1-71794] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Menin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Menin Antibody [Biotin] [NBP1-71794] - Whole cell lysate (40 ug forWB; one 10 cm plate HEK293T cells for IP) from 293T cells. Antibodies: Affinity purified goat anti-Menin used as indicated for IP. Menin was detected by western blot using affinity purified rabbit anti-Menin at0.1 ug/ml for WB. Detection: Chemiluminescence exposed for 1 minute.

Product Discontinued

Menin Antibody [Biotin] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.2% BSA, and 20% Trehalose
• Preservative: 0.09% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml deionized water.

Alternate Names for Menin Antibody [Biotin]

• MEAI
• MEN1
• Menin
• multiple endocrine neoplasia I
• multiple

Background

This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Two variants of the shorter transcript have been identified where alternative splicing affects the CDS. Five variants where alternative splicing takes place in the 5' UTR have also been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEN1
• Entrez:
  • 4221(Human)
  • 17283(Mouse)
  • 29417(Rat)
• Uniprot:
  • NP_000235.2(Human)