Menin Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Menin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Menin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Menin Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Menin
  aa 555-615
  Accession # O00255
• Specificity: Detects human Menin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Menin Antibody [Unconjugated]

• MEAI
• MEN1
• Menin
• multiple endocrine neoplasia I
• multiple

Background

Menin (Multiple endocrine neoplasia type I) is a 75‑78 kDa tumor‑suppressor protein that has no apparent structural relationship to any known protein family. It is ubiquitously expressed and interacts with other molecules, including NF kappa B, FANCD2 and ASK. Menin appears to block gene transcription, promote DNA repair, and inhibit cell proliferation. Human Menin is 615 amino acids (aa) in length. Consistent with its unique molecular taxonomy, it contains no definitive structural module(s). However, it is known to be phosphorylated on Ser399 and Thr599. There are a number of potential splice variants. Individually or in combination, there are deletions of aa 89‑95, 149‑153, 171‑173, 189‑223, and 423‑426. There is also a 50 aa substitution for aa 68‑615, a 66 aa substitution for aa 383‑615, and a two aa substitution for aa 507‑536. Over aa 555‑615, human and mouse Menin are completely identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEN1
• Uniprot:
  • O00255(Human)