Melan-A/MART-1 Antibody (A103)

Images

 
Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted ...read more
Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). ...read more
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
A103
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Melan-A/MART-1 Antibody (A103) Summary

Immunogen
Recombinant human Melan-A/MART-1. [Uniprot# Q16655]
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLANA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 27565163).
Publications
Read Publications using NBP2-46603.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Melan-A/MART-1 Antibody (A103)

  • Antigen LB39-AA
  • Antigen SK29-AA
  • Mart 1 Melan A
  • MART-1
  • MART1MART-1
  • MelanA
  • Melan-A
  • melanoma antigen recognized by T-cells 1
  • MLANA
  • Protein Melan-A

Background

Melan-A/MART-1, also known as MLANA or melanoma antigen recognized by T-cells 1, is expressed exclusively in retina, melanoma, and melanocyte cells. Melan-A interacts with GPR143, ensuring its stability, as well as with PMEL. The interaction between Melan-A and PMEL is crucial to the development of stage II melanosomes, which are organelles that contain and store melanin. Melan-A is found in the membranes of the Golgi apparatus, endoplasmic reticulum, as well as the cell membrane itself. Melan-A's expression on the exterior of melanoma cells are key in the recognition and subsequent lysis of these cells by cytolytic T-Lymphocytes, giving Melan-A a large role in cancer research pertaining to melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Melan-A/MART-1 Antibody (NBP2-46603)(2)

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-46603 Applications Species
Wang Z, Li J, Gao Y et al. Transglutaminase-2 of pancreatic cancer cells assembles a CXCL12-keratin 19-coat that mediates the resistance to immunotherapy bioRxiv
Joshi P, Kooshki M, Aldrich W et al. Expression of natural killer cell regulatory microRNA by uveal melanoma cancer stem cells. Clin. Exp. Metastasis. Aug 26 2016 [PMID: 27565163] (ICC/IF) ICC/IF

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Melan-A/MART-1 Antibody (NBP2-46603) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-46603AF350
Alexa Fluor 405 NBP2-46603AF405
Alexa Fluor 488 NBP2-46603AF488
Alexa Fluor 532 NBP2-46603AF532
Alexa Fluor 594 NBP2-46603AF594
Alexa Fluor 647 NBP2-46603AF647
Alexa Fluor 700 NBP2-46603AF700
Alexa Fluor 750 NBP2-46603AF750
Allophycocyanin/Cy7 NBP2-46603APCCY7
Biotin NBP2-46603B
DyLight 350 NBP2-46603UV
DyLight 405 NBP2-46603V
DyLight 488 NBP2-46603G
DyLight 550 NBP2-46603R
DyLight 594 NBP2-46603DL594
DyLight 650 NBP2-46603C
DyLight 680 NBP2-46603FR
DyLight 755 NBP2-46603IR
FITC NBP2-46603F
HRP NBP2-46603H
Janelia Fluor 549 NBP2-46603JF549
Janelia Fluor 646 NBP2-46603JF646
PE/Atto594 NBP2-46603PEATT594
PE/Cy5.5 NBP2-46603PECY55
PE/Cy7 NBP2-46603PECY7

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Bioinformatics

Gene Symbol MLANA