MEKK4 Antibody (S05-8A5)

Western Blot: MEKK4 Antibody (S05-8A5) [NBP3-19990] - Western blot detection of MEKK4 in HL-60, U251 cell lysates using NBP3-19990 (1:1000 diluted). Predicted band size: 182kDa. Observed band size: 200kDa.

Immunohistochemistry-Paraffin: MEKK4 Antibody (S05-8A5) [NBP3-19990] - Immunohistochemistry of MEKK4 in paraffin-embedded Human lung cancer tissue using NBP3-19990 at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S05-8A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

MEKK4 Antibody (S05-8A5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human MEKK4 (Uniprot # Q9Y6R4)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP3K4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for MEKK4 Antibody (S05-8A5)

• EC 2.7.11.25
• FLJ42439
• MAPKKK4yeast, homolog of
• MEKK 4
• mitogen-activated protein kinase kinase kinase 4

Background

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K4