MED21 Antibody - BSA Free

Western Blot: MED21 Antibody [NBP3-35228] - Western blot analysis of various lysates using MED21 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED21 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED21 Antibody - BSA Free (NBP3-35228) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human MED21 (NP_004255.2).
  
  Sequence:
  MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED21
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MED21 Antibody - BSA Free

• hSrb7
• mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog
• RNA polymerase II complex component SRB7
• RNA polymerase II holoenzyme component SRB7
• RNAPII complex component SRB7
• SRB7 suppressor of RNA polymerase B homolog
• SRB7SRB7 suppressor of RNA polymerase B homolog (yeast)
• SURB7mediator of RNA polymerase II transcription subunit 21

Background

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED21