MeCP2 [p Ser421] Antibody - Azide Free

Western Blot: MeCP2 [p Ser421] Antibody [NBP2-29524] - Western blot of mouse whole brain showing specific immunolabeling of the ~55k truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling is greatly reduced by preadsorption with the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).

• Reactivity: Mu, Rt, Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

MeCP2 [p Ser421] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit MeCP2 [p Ser421] Antibody - Azide Free (NBP2-29524) is a polyclonal antibody validated for use in WB. Anti-MeCP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH. Accession # Q9Z2D6
• Modification: p Ser421
• Marker: Nucleus Marker
• Specificity: Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
• Predicted Species: Human (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MECP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for MeCP2 [p Ser421] Antibody - Azide Free

• AUTSX3
• DKFZp686A24160
• MeCp-2 protein
• mental retardation, X-linked 79
• methyl CpG binding protein 2 (Rett syndrome)
• X-linked 16

Background

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MeCP2 Antibody (NBP2-29524)(1)

Publication using NBP2-29524

• Poon CH, Liu Y, Pak S et al. Prelimbic Cortical Stimulation with L-methionine Enhances Cognition through Hippocampal DNA Methylation and Neuroplasticity Mechanisms Aging and disease 2023-02-01 [PMID: 36818556] (WB, Rat)

Bioinformatics

• Gene Symbol: MECP2
• Entrez:
  • 17257(Mouse)
• Uniprot:
  • Q9Z2D6(Mouse)