MeCP2 [p Ser80] Antibody Summary
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 conjugated to KLH
Specific for the ~75 kDa MECP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:100 - 1:1000
- Immunohistochemistry 1:100
- Immunohistochemistry-Paraffin 1:100
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Whole antisera with 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Alternate Names for MeCP2 [p Ser80] Antibody
- MeCp-2 protein
- mental retardation, X-linked 79
- methyl CpG binding protein 2 (Rett syndrome)
- X-linked 16
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt, Ca, Eq
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Applications: IHC, IHC-P
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ChIP, IP
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Applications: WB, ChIP, Flow, IHC-P, ICC
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Pm
Applications: WB, IHC, IHC-P
Applications: WB, ChIP, IP, PLA
Species: Hu, Mu(-)
Applications: WB (-), ICC/IF, IHC, IHC-P
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Publications for MeCP2 Antibody (NBP1-47558) (0)
There are no publications for MeCP2 Antibody (NBP1-47558).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for MeCP2 Antibody (NBP1-47558) (0)
There are no reviews for MeCP2 Antibody (NBP1-47558).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for MeCP2 Antibody (NBP1-47558) (0)
Additional MeCP2 Products
Bioinformatics Tool for MeCP2 Antibody (NBP1-47558)
Discover related pathways, diseases and genes to MeCP2 Antibody (NBP1-47558). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on MeCP2.