MeCP2 Antibody

Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: MeCP2 Antibody [NB600-1101] - Staining in the nucleus of rat brain tissue (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C6 cells (right) compared to a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MeCP2 Antibody Summary

• Immunogen: Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C
• Localization: Nuclear
• Marker: Nucleus Marker
• Specificity: This detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MECP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 0.5 ug/ml
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MeCP2 Antibody

• AUTSX3
• DKFZp686A24160
• MeCp-2 protein
• mental retardation, X-linked 79
• methyl CpG binding protein 2 (Rett syndrome)
• X-linked 16

Background

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MeCP2 Antibody (NB600-1101)(4)

Publications using NB600-1101

• Lu HE, Yang YC, Chen SM et al. Modeling Neurogenesis Impairment in Down Syndrome with Induced Pluripotent Stem Cells from Trisomy 21 Amniotic Fluid Cells Exp Cell Res 2012-10-04 [PMID: 23041301] (WB, Human)
• Cukier HN, Perez AM, Collins AL et al. Genetic modifiers of MeCP2 function in Drosophila. PLoS Genet. 2008-09-01 [PMID: 18773074]
• LaSalle JM, Goldstine J, Balmer D et al. Quantitative localization of heterogeneous methyl-CpG-binding protein 2 (MeCP2) expression phenotypes in normal and Rett syndrome brain by laser scanning cytometry. Hum Mol Genet. 2001-08-01 [PMID: 11532982]
• Jones PL, Veenstra GJ, Wade PA et al. Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nat Genet. 1998-06-01 [PMID: 9620779]

Bioinformatics

• Gene Symbol: MECP2
• Entrez:
  • 4204(Human)
  • 17257(Mouse)
  • 29386(Rat)
• Uniprot:
  • P51608(Human)