MeCP2 Antibody (4F11) - BSA Free

Western Blot: MeCP2 Antibody [NBP3-05541] - Western blot analysis of tissue lysates using MeCP2 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate. Strong band at about 75kDa in rat and slightly lower in mouse nuclear enriched fractions corresponds to the MeCP2 protein. The same blot was simultaneously probed with chicken pAB to GAP-43 which detects GAP-43 protein in all preparations with apparent molecular weight of 43kDa.

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05541] - Immunofluorescent analysis of rat cerebellum section stained with MeCP2 Antibody at a dilution of 1:500 in red and costained with chicken pAb to calbindin at a dilution of 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels nuclei of neuronal cells to a variable degree, while calbindin is expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq
• Applications: WB, ICC/IF, IHC
• Clone: 4F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MeCP2 Antibody (4F11) - BSA Free Summary

• Description: Novus Biologicals Mouse MeCP2 Antibody (4F11) - BSA Free (NBP3-05541) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant MeCP2 expressed in and purified from E. coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MECP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-5000
  • Western Blot 1:5000-10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MeCP2 Antibody (4F11) - BSA Free

• AUTSX3
• DKFZp686A24160
• MeCp-2 protein
• mental retardation, X-linked 79
• methyl CpG binding protein 2 (Rett syndrome)
• X-linked 16

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MECP2