MDFI Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

MDFI Antibody Summary

Immunogen
Peptide with sequence YQVSGQRPSGCD corresponding to N-Terminus according to NP_005577.1.
Epitope
YQVSGQRPSGCD - N Terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MDFI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 20 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0 kDa band according to NP_005577.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-49886.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for MDFI Antibody

  • I-MF
  • I-mfa
  • inhibitor of MyoD family a
  • MyoD family inhibitor
  • Myogenic repressor I-mf

Background

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDFI Antibody (NBP1-49886)(1)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MDFI
Entrez
Uniprot