MDFI Antibody (3B4) - Azide and BSA Free

Western Blot: MDFI Antibody (3B4) [H00004188-M07] - Analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4. Lane 1: MDFI transfected lysatE (25.029 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MDFI Antibody (3B4) [H00004188-M07] - Analysis of monoclonal antibody to MDFI on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: MDFI Antibody (3B4) [H00004188-M07] - detection against Immunogen (34.69 KDa) .

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MDFI Antibody (3B4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MDFI (NP_005577, 33 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGLEVVTGSTHPAEAAPEEGSLEEAATPMPQGNGPGIPQGLDSTDLDVPTEAVTCQPQGNPLGCTPLLPNDSGHPSEL
• Specificity: MDFI - MyoD family inhibitor (3B4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MDFI
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MDFI Antibody (3B4) - Azide and BSA Free

• I-MF
• I-mfa
• inhibitor of MyoD family a
• MyoD family inhibitor
• Myogenic repressor I-mf

Background

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MDFI
• Entrez:
  • 4188(Human)
• Uniprot:
  • NP_005577(Human)