MD-1/LY86 Antibody (153014)

• Reactivity: Hu
• Applications: WB
• Clone: 153014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MD-1/LY86 Antibody (153014) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MD-1
  Gly21-Ser162
  Accession # O95711
• Specificity: Detects human MD-1 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant mouse MD-1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY86
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MD-1/LY86 Antibody (153014)

• LY86
• Ly-86
• lymphocyte antigen 86
• MD1
• MD-1
• MMD-1
• Protein MD-1
• RP105-associated

Background

MD-1 is a secreted glycoprotein that is associated with RP105 and is required for efficient RP105 cell surface expression and function (1‑4). RP105 is a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (1‑3). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (3).

Human MD-1 cDNA encodes a 162 amino acid (aa) precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 38% and 66% amino acid sequence identity with chicken and mouse MD-1 respectively (1, 2). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. The cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (4, 5). Since MD-1 is also expressed in liver and brain where RP105 is absent, MD-1 may also be associated with other LRR-containing molecules, or have additional functions outside the immune system (5).

1. Miyake, K. et al. (1998) J. Immun. 161:1348.
2. Miura, Y. et al. (1998) Blood 92:2815.
3. Miyake, K. et al. (1995) J. Immunol. 154:3333.
4. Nagai, Y. et al. (2002) Blood 99:1699.
5. Ogata, H. et al. (2000) J. Exp. Med. 192:23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MD-1/LY86 Antibody (MAB925) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/24/2018

Summary

• Application: Western Blot
• Sample Tested: Daudi cell lysate,Daudi cells
• Species: Human
• Lot: HDO012101

Bioinformatics

• Gene Symbol: LY86
• Uniprot:
  • O95711()