MD-1/LY86 Antibody (153014)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
153014
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

MD-1/LY86 Antibody (153014) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MD-1
Gly21-Ser162
Accession # O95711
Specificity
Detects human MD-1 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant mouse MD-1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LY86
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB925 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MD-1/LY86 Antibody (153014)

  • LY86
  • Ly-86
  • lymphocyte antigen 86
  • MD1
  • MD-1
  • MMD-1
  • Protein MD-1
  • RP105-associated

Background

MD-1 is a secreted glycoprotein that is associated with RP105 and is required for efficient RP105 cell surface expression and function (1‑4). RP105 is a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (1‑3). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (3).

Human MD-1 cDNA encodes a 162 amino acid (aa) precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 38% and 66% amino acid sequence identity with chicken and mouse MD-1 respectively (1, 2). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. The cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (4, 5). Since MD-1 is also expressed in liver and brain where RP105 is absent, MD-1 may also be associated with other LRR-containing molecules, or have additional functions outside the immune system (5).

  1. Miyake, K. et al. (1998) J. Immun. 161:1348.
  2. Miura, Y. et al. (1998) Blood 92:2815.
  3. Miyake, K. et al. (1995) J. Immunol. 154:3333.
  4. Nagai, Y. et al. (2002) Blood 99:1699.
  5. Ogata, H. et al. (2000) J. Exp. Med. 192:23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for MD-1/LY86 Antibody (MAB925) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB925:
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Western Blot MD-1/LY86 MAB925
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5
reviewed by:
Verified Customer
WB Human 10/24/2018
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Summary

ApplicationWestern Blot
Sample TestedDaudi cell lysate,Daudi cells
SpeciesHuman
LotHDO012101

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Verified Customer
10/24/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol LY86
Uniprot