MCM7 Antibody (2068B) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MCM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCM7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MCM7 was detected in immersion fixed paraffin-embedded sections of human mesothelioma tissue using Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human MCM7 Monoclonal Antibody (Catalog # MAB9217, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, IHC, Flow, ICC/IF
• Clone: 2068B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MCM7 Antibody (2068B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human MCM7
  Gly188-Ala328
  Accession # P33993
• Specificity: Detects human MCM7 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCM7 Antibody (2068B) [Unconjugated]

• CDC47 homolog
• CDC47
• CDC47P85MCM
• DNA replication licensing factor MCM7
• EC 3.6.4.12
• homolog of S. cerevisiae Cdc47
• MCM2
• MCM2PNAS146
• MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
• MCM7
• minichromosome maintenance complex component 7
• minichromosome maintenance deficient (S. cerevisiae) 7
• minichromosome maintenance deficient 7
• P1.1-MCM3
• P1CDC47
• P85MCM
• PNAS146
• PPP1R104
• Protein Phosphatase 1

Background

MCM7/minichromosome maintenance complex component 7, is a highly conserved mini-chromosome maintenance protein, with ATP binding, DNA helicase activity and single-stranded DNA binding. MCM7 forms a hexameric protein complex with MCM2, 4  and 6 proteins that has DNA helicase activity essential for the initiation of gene replication.  MCM7 also binds to RAD17 to mediate ATR recruitment to damaged DNA. MCM7 expression is repressed in quiescent cells but induced in late G1 to S phase by growth factor stimulation.  It is up-regulated in many cancers including colorectal , T cell lymphomas, glioblastoma, and has prognostic value for hepatocellular carcinoma and gastric adenocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P33993()