| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35353-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85722-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38168-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55587-25ul | |||
![Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NB100-79778-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-NB100-79778-img0010.jpg)
![Immunoprecipitation: MCM5 Antibody [NBP3-35353] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411364612.jpg "Immunoprecipitation: MCM5 Antibody [NBP3-35353] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.")
![Western Blot: MCM5 Antibody [NBP3-35353] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411381225.jpg)
![Immunohistochemistry: MCM5 Antibody [NBP3-35353] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411343348.jpg)
![Immunohistochemistry: MCM5 Antibody [NBP3-35353] - Immunohistochemistry analysis of paraffin-embedded Human appendix using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411343375.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-35353] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35353_rabbit-mcm5-pab-1012202411334043.jpg)
![Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Western-Blot-NBP3-16529-img0003.jpg "Western Blot: MCM5 Antibody (4P1Q5) [NBP3-16529] - Western blot analysis of extracts of various cell lines, using MCM5 Rabbit mAb (NBP3-16529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded human colon using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0002.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunohistochemistry of paraffin-embedded rat testis using MCM5 Rabbit mAb (NBP3-16529) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunohistochemistry-Paraffin-NBP3-16529-img0001.jpg)
![Immunoprecipitation: MCM5 Antibody (4P1Q5) [NBP3-16529] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug MCM5 antibody (NBP3-16529). Western blot was performed from the immunoprecipitate using MCM5 antibody (NBP3-16529) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM5-Antibody-4P1Q5-Immunoprecipitation-NBP3-16529-img0004.jpg)
![Western Blot: MCM5 Antibody [NBP1-85722] - Analysis in control (vector only transfected HEK293T lysate) and MCM5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MCM5-Antibody-Western-Blot-NBP1-85722-img0008.jpg "Western Blot: MCM5 Antibody [NBP1-85722] - Analysis in control (vector only transfected HEK293T lysate) and MCM5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722] - Staining in human bone marrow and pancreas tissues using anti-MCM5 antibody. Corresponding MCM5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-Paraffin-NBP1-85722-img0006.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-Paraffin-NBP1-85722-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM5 Antibody [NBP1-85722] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunohistochemistry-Paraffin-NBP1-85722-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-91220241581241.jpg "Immunocytochemistry/ Immunofluorescence: MCM5 Antibody [NBP3-38168] - Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: MCM5 Antibody [NBP3-38168] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MCM5 antibody. Western blot was performed from the immunoprecipitate using MCM5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202415111841.jpg)
![Western Blot: MCM5 Antibody [NBP3-38168] - Western blot analysis of various lysates using MCM5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38168_rabbit-mcm5-pab-912202414112858.jpg)
![Immunohistochemistry:[NBP3-29204]-Detection of human MCM5 by immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-MCM5.](http://images.novusbio.com/fullsize/nbp3-29204_rabbit-mcm5-pab-23120251225662.jpg "Immunohistochemistry:[NBP3-29204]-Detection of human MCM5 by immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-MCM5.")
![Immunocytochemistry/Immunofluorescence: MCM5 Antibody [NBP2-55587] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MCM5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55587-img0001.jpg "Immunocytochemistry/Immunofluorescence: MCM5 Antibody [NBP2-55587] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Western Blot: MCM5 Overexpression Lysate (Adult Normal) [NBL1-12954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCM5.](http://images.novusbio.com/fullsize/MCM5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12954-img0002.jpg "Western Blot: MCM5 Overexpression Lysate (Adult Normal) [NBL1-12954] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCM5.")
![ELISA: Human MCM5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39479] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39479_human-mcm5-ready-to-use-elisa-kit-colorimetric-2110202415384094.png "ELISA: Human MCM5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39479] - Standard Curve Reference")
![ELISA: Human MCM5 ELISA Kit (Colorimetric) [NBP3-39478] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39478_human-mcm5-elisa-kit-colorimetric-2110202415343921.png "ELISA: Human MCM5 ELISA Kit (Colorimetric) [NBP3-39478] - Standard Curve Reference")