![Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0012.jpg "Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit MCM3 Antibody - BSA Free (NB100-289) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-MCM3 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MCM3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot and immunoprecipitation. Suggested working dilutions: Western Blot - 1: 2,000-1:10,000 Immunohistochemistry/Immunocytochemistry - ND Immunoprecipitation - 2 to 5 ug/mg lysate** *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-288 (MCM2 antibody) to IP the MCM complex |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Yongkang Yang |
WB | Human | 11/28/2024 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-289-img0010.jpg "Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0002.jpg "Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.")
![Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-289_rabbit-polyclonal-mcm3-antibody-western-blot-2122024155814..jpg "Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
