MCM2 [p Ser27] Antibody (SR1107) - BSA Free

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Western Blot: MCM2 [p Ser 27] Antibody (SR1107) [NBP3-27825] - Western blot analysis of (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SR1107
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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MCM2 [p Ser27] Antibody (SR1107) - BSA Free Summary

Description
Novus Biologicals Rabbit MCM2 [p Ser27] Antibody (SR1107) - BSA Free (NBP3-27825) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human MCM2 (Uniprot #: P49736)
Modification
p Ser27
Specificity
Detects endogenous levels of total MCM2
Source
HEK293
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MCM2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 150mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MCM2 [p Ser27] Antibody (SR1107) - BSA Free

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Background

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MCM2