Mcl-1 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Mcl-1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Mcl-1
  Val147-Gly219
  Accession # Q07820
• Specificity: Detects human Mcl-1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mcl-1 Antibody [Alexa Fluor® 488]

• BCL2L3
• bcl2-L-3
• BCL2L3MGC104264
• Bcl-2-like protein 3
• Bcl-2-related protein EAT/mcl1
• EAT
• induced myeloid leukemia cell differentiation protein Mcl-1
• Mcl1
• Mcl-1
• mcl1/EAT
• MCL1-ES
• MCL1L
• MCL1S
• MGC1839
• myeloid cell leukemia ES
• myeloid cell leukemia sequence 1 (BCL2-related)
• TM

Background

MCL-1 (induced Myeloid Cell Leukemia differentiation protein 1; also Bcl2L3 and mcl1/EAT) is a 40-45 kDa member of the Bcl-2 family of proteins. It is widely expressed (in B cells, T cells, neutrophils and fibroblasts) and classified as a prosurvival Bcl-2 family member. Functionally, full-length MCL-1 sequesters the proapoptotic proteins Bak and Bax, rendering them inactive. It also delays cell-cycle progression by interacting with CDK1, CHK1 and PCNA. Human MCL-1 is a likely a 350 amino acid (aa) type II transmembrane protein. It contains a large cytoplasmic region (aa 1-327) plus a very short 2 aa C-terminal luminal segment. The cytoplasmic region has multiple domains, including a PEST (Pro/Glu/Ser/Thr)-like segment (aa 104-175), four ubiquitination sites, at least six utilized phosphorylation sites, and three Bcl2-like homology domains (aa 209-223; 252-272; 304-319). MCL-1 is known to form homodimers. There is one splice variant. It is 32-33 kDa in size and contains a 42 aa substitution for aa 230-350. This short form heterodimerizes with full-length MCL-1, rendering it incapable of interacting with Bak and Bax. MCL-1 also undergoes caspase processing. Cleavage after Asp127 generates 17 and 28-30 kDa fragments, while cleavage after Asp 157 generates 21 and 23-25 kDa fragments. These fragments give rise to a proapoptotic environment. Over aa 147-219, human MCL-1 shares 74% aa sequence identity with mouse Mcl-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.