![Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.](http://images.novusbio.com/fullsize2/MBP-Antibody-NBP2-50035-img0002.jpg "Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Chicken |
| Conjugate | Unconjugated |
| Immunogen | Purified myelin basic protein isolated from bovine brain. [UniProt# P02687] |
| Isotype | IgY |
| Clonality | Polyclonal |
| Host | Chicken |
| Gene | MBP |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, this antibody recognizes all four CNS isotypes of rat MBP with bands at 21.5 kDa, 18.5kDa, 17kDa and 14kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | PBS |
| Preservative | 5mM Sodium Azide |
| Purity | Ammonium sulfate precipitation |
Secondary Antibodies |
Isotype Controls |
Diseases for MBP Antibody (NBP2-50035)Discover more about diseases related to MBP Antibody (NBP2-50035).
| Pathways for MBP Antibody (NBP2-50035)View related products by pathway.
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PTMs for MBP Antibody (NBP2-50035)Learn more about PTMs related to MBP Antibody (NBP2-50035).
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| Gene Symbol | MBP |
![Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize2/MBP-Antibody-NBP2-50035-img0001.jpg "Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.")
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](http://images.novusbio.com/fullsize/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. Image from verified customer review.](http://images.novusbio.com/fullsize2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0003.jpg)
![Western Blot: MOG Antibody [H00004340-B01P] - Analysis of MOG expression in rat brain.](http://images.novusbio.com/fullsize2/MOG-Antibody-Western-Blot-H00004340-B01P-img0003.jpg)
![Western Blot: MOG Antibody [H00004340-B01P] - Analysis of MOG expression in transfected 293T cell line by MOG polyclonal antibody. Lane 1: MOG transfected lysate(32.56 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/MOG Antibody-Western Blot-H00004340-B01P-img0004.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Border of a demyelinated lesion. Image from a verified customer review.](http://images.novusbio.com/fullsize2/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0013.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0010.jpg)
![Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/Myelin-PLP-Antibody-Immunohistochemistry-Paraffin-NBP1-87781-img0011.jpg)
![Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - HepG2 cell lysate, Antibody Titration: 2.0ug/ml](http://images.novusbio.com/fullsize2/PTHLH-PTHrP-Antibody-Western-Blot-NBP1-59322-img0001.jpg)
![Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize2/PTHLH-PTHrP-Antibody-Immunohistochemistry-Paraffin-NBP1-59322-img0003.jpg)
![Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary, Antibody Dilution: 1 : 2000.](http://images.novusbio.com/fullsize2/PTHLH-PTHrP Antibody-Western Blot-NBP1-59322-img0004.jpg)
![Western Blot: PDXP Antibody [NBP2-27461] - 0.1 ug/ml staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/PDXP-Antibody-Western-Blot-NBP2-27461-img0004.jpg)
![Immunohistochemistry-Paraffin: PDXP Antibody [NBP2-27461] - 5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize2/PDXP-Antibody-Immunohistochemistry-Paraffin-NBP2-27461-img0005.jpg)
![Western Blot: PDXP Antibody [NBP2-27461] - NBP2-27461 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/PDXP-Antibody-Western-Blot-NBP2-27461-img0003.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining of A-431 cells using the Anti-LAMC2 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Immunocytochemistry-Immunofluorescence-NBP2-42388-img0025.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Immunohistochemistry-Paraffin-NBP2-42388-img0023.jpg)
![Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12](http://images.novusbio.com/fullsize2/ERK2 Antibody (SZ25-01)-Western Blot-NBP2-67360-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunohistochemistry-Paraffin-NBP2-67360-img0007.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Western Blot: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of CNPase on mouse brain lysates using anti-CNPase antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/CNPase-Antibody-(JF10-25)-Western-Blot-NBP2-66986-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/CNPase Antibody (JF10-25)-Immunocytochemistry-Immunofluorescence-NBP2-66986-img0007.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of paraffin-embedded rat brain tissue using anti-CNPase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/CNPase-Antibody-(JF10-25)-Immunohistochemistry-Paraffin-NBP2-66986-img0005.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/fullsize2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19371-img0005.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/fullsize2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0003.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
![Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize2/SGSM3-Antibody-Western-Blot-NBP2-13304-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize2/SGSM3 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/SGSM3-Antibody-Immunohistochemistry-Paraffin-NBP2-13304-img0001.jpg)
