MBP Antibody (932908) [Unconjugated]

MBP was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

MBP was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15‑22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces, cytoplasm, and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

MBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clone: 932908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MBP Antibody (932908) [Unconjugated] Summary

• Immunogen: Purified MBP from bovine brain
  Accession # P02686
• Specificity: Detects human, mouse, and rat MBP
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBP

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 0.1-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MBP Antibody (932908) [Unconjugated]

• Hmbpr
• MBP
• MGC99675
• mld
• Myelin A1 protein
• myelin basic protein
• Myelin membrane encephalitogenic protein
• shi

Background

Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBP Antibody (MAB42282)(1)

Publication using MAB42282

• T Liang, Q Chen, Q Li, R Li, J Tang, R Hu, J Zhong, H Ge, X Liu, F Hua 5-HT1a activation in PO/AH area induces therapeutic hypothermia in a rat model of intracerebral hemorrhage Oncotarget, 2017;8(43):73613-73626. 2017 [PMID: 29088731] (IHC, Rat)

Bioinformatics

• Gene Symbol: MBP
• Uniprot:
  • P02686()