MBL Antibody (3B6) - BSA Free

Immunohistochemistry-Paraffin: MBL Antibody (3B6) - BSA Free [NBP2-23582] - Paraffin-embedded human cancerous liver tissue

Immunohistochemistry-Paraffin: MBL Antibody (3B6) - BSA Free [NBP2-23582] - Paraffin-embedded human liver tissue

Immunohistochemistry-Paraffin: MBL Antibody (3B6) - BSA Free [NBP2-23582] - Paraffin-embedded human liver tissue

ELISA: MBL Antibody (3B6) - BSA Free [NBP2-23582] - The calibration curve of a sandwich assay for MBL using NBP2-23582 as the capture antibody and NBP2-23581as the biotinylated detection antibody.

Product Discontinued

MBL Antibody (3B6) - BSA Free Summary

• Immunogen: MBL purified from human donor plasma and adsorbed onto aluminum hydroxide
• Epitope: The epitope is on the head-neck region of the MBL protein chain. Prior binding of the antibody is thought to block binding to carbohydrate. The epitope differs from that of Clone 10 and Clone 11.
• Specificity: Clone 3B6 is specific for MBL from human serum or plasma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MBL2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Clone 3B6 is selective for normally oligomerized MBL when used as detection antibody in sandwich ELISA with Clone 3B6 coat.In Western blotting, Clone 3B6 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa.Clone 3B6 is also well suited for immunohistochemistry on human tissue samples, frozen or paraffin embedded, from liver and brain.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MBL Antibody (3B6) - BSA Free

• COLEC1
• COLEC1collectin-1
• Collectin-1
• HSMBPC
• Mannan-binding protein
• mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• mannose-binding lectin (protein C) 2, soluble
• Mannose-binding lectin
• mannose-binding protein C
• MBL
• MBL2
• MBL2D
• MBLmannan-binding lectin
• MBP
• MBP1
• MBP1mannose-binding lectin 2, soluble (opsonic defect)
• MBP-C
• MGC116832
• MGC116833

Background

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MBL2