MBD4 Antibody

Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.

Western Blot: MBD4 Antibody [NBP2-24393] - Analysis of MBD4 in HL60 cell lysate probed with MBD4 antibody.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MBD4 Antibody Summary

• Description: Novus Biologicals Rabbit MBD4 Antibody (NBP2-24393) is a polyclonal antibody validated for use in WB and ChIP. Anti-MBD4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-300 and 337-374 of human MBD4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 15689352)
  • Western Blot 2-4 ug/ml

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17546630).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for MBD4 Antibody

• EC 3.2.2.-
• G/T mismatch glycosylase
• G/U mismatch glycosylase
• MBD4
• MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics
• methyl-CpG binding domain protein 4
• methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase
• Methyl-CpG-binding endonuclease 1
• Methyl-CpG-binding protein MBD4
• Mismatch-specific DNA N-glycosylase
• putative methyl-CpG binding protein

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBD4 Antibody (NBP2-24393)(2)

Publications using NBP2-24393

• Ruddock-D'Cruz NT, Xue J, Wilson KJ et al. Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development. Mol Reprod Dev. 2008-01-01 [PMID: 17546630] (WB, Mouse)
  
  Details:
  IF [bovine embryos (Fig 3) and mouse embryos (Fig 4)] and for WB [bovine heart, (supplementary data)] 1. MBD1 mAb (IMG-306) 2. MBD3/MBD2 mAb (IMG-296A) 3. MBD4 pAb (IMG-285).
• Thatcher KN, Peddada S, Yasui DH, Lasalle JM. Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples. Hum Mol Genet. 2005-03-15 [PMID: 15689352] (Chemotaxis)
  
  Details:
  ChIP (Fig 6): SH-SY5Y cells

Bioinformatics

• Gene Symbol: MBD4