![Immunocytochemistry/Immunofluorescence: MAST4 Antibody [NBP1-83017] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/MAST4 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83017-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAST4 Antibody [NBP1-83017] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGPVPAEHRQGGGDFRRAPAPGTLQDGLCHS |
| Specificity | Specificity of human MAST4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAST4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for MAST4 Antibody (NBP1-83017)Discover more about diseases related to MAST4 Antibody (NBP1-83017).
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Research Areas for MAST4 Antibody (NBP1-83017)Find related products by research area.
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| Gene Symbol | MAST4 |
![Immunohistochemistry-Paraffin: MAST4 Antibody [NBP1-83017] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize2/MAST4-Antibody-Immunohistochemistry-Paraffin-NBP1-83017-img0004.jpg "Immunohistochemistry-Paraffin: MAST4 Antibody [NBP1-83017] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.")
![Western Blot: CLASP1 Antibody [NBP2-15910] - A: Mouse Brain.](http://images.novusbio.com/fullsize2/CLASP1-Antibody-Western-Blot-NBP2-15910-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CLASP1 Antibody [NBP2-15910] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/CLASP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15910-img0005.jpg)
![Immunohistochemistry-Paraffin: CLASP1 Antibody [NBP2-15910] - Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/CLASP1-Antibody-Immunohistochemistry-Paraffin-NBP2-15910-img0006.jpg)
![Immunohistochemistry-Paraffin: MAST1 Antibody [NBP2-62681] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/MAST1-Antibody-Immunohistochemistry-Paraffin-NBP2-62681-img0002.jpg)
![Immunohistochemistry-Paraffin: MAST1 Antibody [NBP2-62681] - Analysis in human cerebral cortex and pancreas tissues using Anti-MAST1 antibody. Corresponding MAST1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/MAST1-Antibody-Immunohistochemistry-Paraffin-NBP2-62681-img0001.jpg)
![Immunohistochemistry-Paraffin: MAST1 Antibody [NBP2-62681] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize2/MAST1 Antibody-Immunohistochemistry-Paraffin-NBP2-62681-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAST3 Antibody [NBP1-82993] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/MAST3 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82993-img0004.jpg)
![Immunohistochemistry-Paraffin: MAST3 Antibody [NBP1-82993] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize2/MAST3-Antibody-Immunohistochemistry-Paraffin-NBP1-82993-img0002.jpg)
![Western Blot: MASTL Antibody [NBP1-22987] - Detection of Human MASTL by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MASTL antibody NBP1-22987 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/MASTL-Antibody-Western-Blot-NBP1-22987-img0007.jpg)
![Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-22987] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MASTL used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/MASTL-Antibody-Immunohistochemistry-Paraffin-NBP1-22987-img0008.jpg)
![Western Blot: MASTL Antibody [NBP1-22987] - Whole cell lysate from HeLa and 293T cells. MASTL was also immunoprecipitated by another rabbit anti-MASTL antibody.](http://images.novusbio.com/fullsize2/MASTL-Antibody-Western-Blot-NBP1-22987-img0002.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Western blot analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Western-Blot-NBP2-22194-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg)
![Immunohistochemistry-Paraffin: TTF1 / NKX2.1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/fullsize2/TTF1---NKX2.1-Antibody-(8G7G3-1-+-NX2.1-690)-Immunohistochemistry-Paraffin-NBP2-44501-img0001.jpg)
![Immunohistochemistry-Paraffin: TTF1 / NKX2.1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)](http://images.novusbio.com/fullsize2/TTF1 - NKX2.1 Antibody (8G7G3-1 + NX2.1-690)-Immunohistochemistry-Paraffin-NBP2-44501-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-83430] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.](http://images.novusbio.com/fullsize2/Reduced-Folate-Carrier-SLC19A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83430-img0004.jpg)
![Immunohistochemistry-Paraffin: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-83430] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize2/Reduced Folate Carrier-SLC19A1 Antibody-Immunohistochemistry-Paraffin-NBP1-83430-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CDCA7 Antibody [NBP1-82224] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/CDCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82224-img0005.jpg)
![Immunohistochemistry-Paraffin: CDCA7 Antibody [NBP1-82224] - Staining of human lymph node shows strong nuclear and cytoplasmic positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize2/CDCA7-Antibody-Immunohistochemistry-Paraffin-NBP1-82224-img0007.jpg)
![Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-MCM6 antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize2/MCM6-Antibody-Western-Blot-NBP1-82642-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: MCM6 Antibody [NBP1-82642] - Staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.](http://images.novusbio.com/fullsize2/MCM6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82642-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining in human lymph node and liver tissues using anti-MCM6 antibody. Corresponding MCM6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/MCM6-Antibody-Immunohistochemistry-Paraffin-NBP1-82642-img0009.jpg)
![Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.](http://images.novusbio.com/fullsize2/MRP1 Antibody (IU2H10)-Western Blot-NB400-156-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize2/MRP1-Antibody-(IU2H10)-Immunocytochemistry-Immunofluorescence-NB400-156-img0007.jpg)
![Western Blot: RNA polymerase I termination factor Antibody [NBP2-38740] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/RNA-polymerase-I-termination-factor-Antibody-Western-Blot-NBP2-38740-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: RNA polymerase I termination factor Antibody [NBP2-38740] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize2/RNA polymerase I termination factor Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38740-img0004.jpg)
![Immunohistochemistry: RNA polymerase I termination factor Antibody [NBP2-38740] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize2/RNA-polymerase-I-termination-factor-Antibody-Immunohistochemistry-NBP2-38740-img0002.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0004.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")