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MAST4 Antibody

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Immunocytochemistry/Immunofluorescence: MAST4 Antibody [NBP1-83017] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunocytochemistry/ Immunofluorescence: MAST4 Antibody [NBP1-83017] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: MAST4 Antibody [NBP1-83017] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MAST4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGPVPAEHRQGGGDFRRAPAPGTLQDGLCHS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAST4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAST4 Protein (NBP1-83017PEP)
Publications
Read Publication using NBP1-83017.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAST4 Antibody

  • KIAA0303

Background

Microtubule-associated serine/threonine-protein kinase 4 or MAST4 is involved with protein phosphorylation and can be found within the cytoplasm. Belonging to the AGC Ser/Thr protein kinase family, MAST4 are cytoskeletal kinases associated with protein serine/threonine kinase activity; magnesium ion binding and ATP binding. Popular protein domains for MAST3 include PDZ, protein kinase, AGC-kinase C-terminal domain and domain of unknown function (DUF1908). MAST4 is also known to interact with SMAD1 and MYC. Diseases connected to MAST4 consist of Down syndrome and cystic lymphangioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: CLASP1 Antibody [NBP2-15910] - Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using antibody at 1:100 dilution.
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Western Blot: MAST1 Antibody [NBP2-17228] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.
Western Blot: MAST1 Antibody [NBP2-17228] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MAST1 antibody [N2N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Immunohistochemistry-Paraffin: MAST3 Antibody [NBP1-82993] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
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Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
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Immunocytochemistry/Immunofluorescence: MASTL Antibody [NBP1-83680] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: MASTL Antibody [NBP1-83680] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
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Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, Cytokeratin 5, CK6 and CK14. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128096) licensed under a CC-BY licence.
Simple Western: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Simple Western lane view shows a specific band for Cytokeratin 5 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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AF231
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Immunohistochemistry-Paraffin: RNA polymerase I termination factor Antibody [NBP2-38740] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RNA polymerase I termination factor Antibody [NBP2-38740] - Staining of human liver shows weak nuclear positivity in hepatocytes.
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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MAST4 Antibody
NBP1-83017
MAST4 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for MAST4 Antibody (NBP1-83017)(1)


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Bioinformatics

Gene Symbol MAST4