ATAD2 Antibody

Images

 
Genetic Strategies: Western Blot: ATAD2 Antibody [NBP1-84123] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATAD2 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody [NBP1-84123] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining in human testis and epididymis tissues using anti-ATAD2 antibody. Corresponding ATAD2 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human epididymis shows low expression as expected.
Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP1-84123] - Staining of human testis shows high expression.
Simple Western: ATAD2 Antibody [NBP1-84123] - Simple Western lane view shows a specific band for ATAD2 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATAD2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSKYAPSYYHVMPKQNSTLVGDKRSDPEQNEKLKTPSTPVACSTPAQLKRKIRKKSNWYLGTIKKRRKISQAKDDSQNAIDHK
Specificity
Specificity of human ATAD2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATAD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
ATAD2 Lysate (NBP2-64885)
Control Peptide
ATAD2 Protein (NBP1-84123PEP)
Publications
Read Publications using
NBP1-84123 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (82%), Rat (82%). Reactivity reported in scientific literature (PMID: 23393560)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATAD2 Antibody

  • AAA nuclear coregulator cancer-associated protein
  • ANCCA
  • ANCCAMGC131938
  • ATAD2
  • ATPase family AAA domain-containing protein 2
  • ATPase family, AAA domain containing 2
  • CT137
  • DKFZp667N1320
  • EC 3.6.1.3
  • MGC142216
  • MGC29843
  • MGC5254
  • PRO2000

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATAD2 Antibody (NBP1-84123)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATAD2
Entrez
Uniprot