Matrin 3 Antibody

Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - Detection of Human and Mouse Matrin 3 on HeLa whole cell lysate using NB100-1761. Matrin 3 was also immunoprecipitated using another rabbit anti-Matrin 3 antibody and NB100-1559.

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated

Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761] - Section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: Matrin 3 Antibody [NB100-1761] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121) licensed under a CC-BY licence.

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of f longitudinal sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - IP of human fibroblasts. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Matrin 3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 800 and the C-terminus (residue 847) of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MATR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Matrin 3 Antibody

• distal 2
• DKFZp686K0542
• DKFZp686K23100
• matrin 3
• matrin-3
• vocal cord and pharyngeal weakness with distal myopathy

Background

Matrin 3 is a nuclear matrix protein that has been observed to mediate multiple cellular processes. Matrin 3 can function to withhold defective RNAs within the nucleus by anchoring them to the nuclear matrix and modulate the promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). Matrin 3 has also been demonstrated to be the main PKA substrate following NMDA receptor activation in NMDA-induced neuronal cell death. Alternate names for Matrin 3 include MATR3, MGC9105, KIAA0723, DKFZp686K0542, and DKFZp686K23100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Matrin 3 Antibody (NB100-1761)(11)

Publications using NB100-1761

• Moloney C, Rayaprolu S, Howard J et al. Analysis of spinal and muscle pathology in transgenic mice overexpressing wild-type and ALS-linked mutant MATR3 Acta Neuropathol Commun Dec 19 2018 [PMID: 30563574] (WB, Mouse)
• Marangi G, Lattante S, Doronzio PN et al. Matrin 3 variants are frequent in Italian ALS patients. Neurobiol Aging 2017 Jan [PMID: 28029397]
• Moloney C, Rayaprolu S, Howard J et al. Transgenic mice overexpressing the ALS-linked protein Matrin 3 develop a profound muscle phenotype. Acta Neuropathol Commun. Nov 18 2016 [PMID: 27863507] (WB, Mouse)
• Rayaprolu S, D'Alton S, Crosby K et al. Heterogeneity of Matrin 3 in the developing and aging murine central nervous system. J Comp Neurol 2016 Oct 01 [PMID: 26878116] (Mouse)
• Yarosh CA, Tapescu I, Thompson MG et al. TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells. Nucleic Acids Res. 2015 Aug 10 [PMID: 26261210] (WB, Human)
• Moldovan JB, Moran JV. The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet. 2015 May 01 [PMID: 25951186] (WB, ICC/IF, Human)
• Depreux FF, Puckelwartz MJ, Augustynowicz A et al. Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations. Hum. Mol. Genet. 2015 May 06 [PMID: 25948554] (IP, WB, Mouse)
• Lee YW, Terranova C, Birkaya B et al. A novel nuclear FGF Receptor-1 partnership with retinoid and Nur receptors during developmental gene programming of embryonic stem cells. J Cell Biochem 2012 Sep [PMID: 22539306]
• Salton M, Elkon R, Borodina T et al. Matrin 3 binds and stabilizes mRNA. PLoS One 2011 [PMID: 21858232]
• Erazo A, Yee MB, Banfield BW et al. The alphaherpesvirus US3/ORF66 protein kinases direct phosphorylation of the nuclear matrix protein matrin 3. J Virol 2011 Jan [PMID: 20962082]
• Schessl J, Zou Y, McGrath MJ et al. Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy. J Clin Invest 2008 Mar [PMID: 18274675]

Review for Matrin 3 Antibody (NB100-1761) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Jennifer Fox IP Human 09/12/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Human fibroblasts
• Species: Human
• Lot: A1
• Comments: Rabbit IgG at 2ug:500ug lysate as negative control (Lane 2). 40ug of input as positive control (Lane 1)Cells lysed in 1X RIPA containing phosphatase and protease inhibitors.

Blocking

• Blocking Details: NA

Primary Anitbody

• Dilution Ratio: 2ug:500ug lysate o/n 4C for IP

Details

• Immunoprecipitation Details: Protein A beads, 50ul slurry. 2H pre-clear, 2ug AB:500ug Lysate o/n 4C, 2H Beads/Lysate/AB 4C. 4 washes in 1X Ripa 4C
• Elution Description: 1X LDS buffer, no DTT 10 minutes room temperature

Comments

• Comments: Rabbit IgG at 2ug:500ug lysate as negative control (Lane 2). 40ug of input as positive control (Lane 1)Cells lysed in 1X RIPA containing phosphatase and protease inhibitors.

Bioinformatics

• Gene Symbol: MATR3
• Entrez:
  • 9782(Human)
  • 17184(Mouse)
• Uniprot:
  • P43243(Human)