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Matrin 3 Antibody

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 (1 Review)
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Immunocytochemistry/Immunofluorescence: Matrin 3 Antibody [NB100-1761] - Section of human breast carcinoma.
Immunocytochemistry/ Immunofluorescence: Matrin 3 Antibody [NB100-1761] - Section of human breast carcinoma.
Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent ...read more
Immunocytochemistry/ Immunofluorescence: Matrin 3 Antibody [NB100-1761] - Section of human breast carcinoma.
Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 ...read more
Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - IP of human fibroblasts. Image from verified customer review.
Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - Detection of Human and Mouse Matrin 3 on HeLa whole cell lysate using NB100-1761. Matrin 3 was also immunoprecipitated using another rabbit anti-Matrin 3 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Matrin 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 800 and the C-terminus (residue 847) of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MATR3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1761 in the following applications:
Publications
Read Publications using
NB100-1761 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Matrin 3 Antibody

  • distal 2
  • DKFZp686K0542
  • DKFZp686K23100
  • matrin 3
  • matrin-3
  • vocal cord and pharyngeal weakness with distal myopathy

Background

Matrin 3 is a nuclear matrix protein that has been observed to mediate multiple cellular processes. Matrin 3 can function to withhold defective RNAs within the nucleus by anchoring them to the nuclear matrix and modulate the promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). Matrin 3 has also been demonstrated to be the main PKA substrate following NMDA receptor activation in NMDA-induced neuronal cell death. Alternate names for Matrin 3 include MATR3, MGC9105, KIAA0723, DKFZp686K0542, and DKFZp686K23100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. This image was submitted via customer review.
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. This image was submitted by customer review.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB
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Western Blot: SFPQ Antibody [NBP2-20329] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody diluted by 1:10000.
Immunocytochemistry/Immunofluorescence: SFPQ Antibody [NBP2-20329] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20329] - Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
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Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
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Western Blot: APH1A Antibody [NB100-74360] - Analysis of mouse brain and kidney extracts.
Immunocytochemistry/Immunofluorescence: APH1A Antibody [NB100-74360] - Staining of APH-1 in human KNS-42 glioma cells.
(2 Publications)
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
hnRNP U Antibody
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Western Blot: hnRNP U Antibody [NB100-2134] - Detection of Human and Mouse hnRNP-U on HeLa whole cell lysate using NB100-2135.
Immunohistochemistry: hnRNP U Antibody [NB100-2134] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-U used at a dilution of 1:200 (1ug/ml). Detection: DAB
(1 Publication)
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Immunocytochemistry/Immunofluorescence: ZNF638 Antibody [NBP1-82730] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: ZNF638 Antibody [NBP1-82730] - Staining of human nasopharynx shows strong nuclear positivity in respiratory epithelial cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP‑rp1/IGFBP‑7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IGFBP‑rp1/IGFBP‑7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot: Myotilin Antibody [NBP2-38361] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skeletal muscle shows high expression.
Species: Hu
Applications: WB, IHC, IHC-P
DDX6 Antibody
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Western Blot: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Immunohistochemistry: DDX6 Antibody [NB200-191] - Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image was submitted via customer Review.
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Western Blot: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Western Blot: FKBP51/FKBP5 Antibody [NBP1-84676] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human cell line HEK 293 shows localization to nucleoplasm.
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Western blot shows lysates of human skeletal muscle tissue and human heart (atrium) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications: WB, IHC
ATM Antibody
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Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using NB100-104. (30 sec. exposure)
Immunocytochemistry/Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton-X100. Then cells were incubated with anti-ATM (NB100-104) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone.
(2 Reviews)
(80 Publications)
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Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
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Publications for Matrin 3 Antibody (NB100-1761)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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Showing Publications 1 - 10 of 10.
Publications using NB100-1761 Applications Species
Marangi G, Lattante S, Doronzio PN et al. Matrin 3 variants are frequent in Italian ALS patients. Neurobiol Aging 2017 Jan [PMID: 28029397]
Moloney C, Rayaprolu S, Howard J et al. Transgenic mice overexpressing the ALS-linked protein Matrin 3 develop a profound muscle phenotype. Acta Neuropathol Commun. Nov 18 2016 [PMID: 27863507] (WB, Mouse) WB Mouse
Rayaprolu S, D'Alton S, Crosby K et al. Heterogeneity of Matrin 3 in the developing and aging murine central nervous system. J Comp Neurol 2016 Oct 01 [PMID: 26878116] (Mouse) Mouse
Yarosh CA, Tapescu I, Thompson MG et al. TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells. Nucleic Acids Res. 2015 Aug 10 [PMID: 26261210] (WB, Human) WB Human
Moldovan JB, Moran JV. The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition. PLoS Genet. 2015 May 01 [PMID: 25951186] (WB, ICC/IF, Human) WB, ICC/IF Human
Depreux FF, Puckelwartz MJ, Augustynowicz A et al. Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations. Hum. Mol. Genet. 2015 May 06 [PMID: 25948554] (IP, WB, Mouse) IP, WB Mouse
Lee YW, Terranova C, Birkaya B et al. A novel nuclear FGF Receptor-1 partnership with retinoid and Nur receptors during developmental gene programming of embryonic stem cells. J Cell Biochem 2012 Sep [PMID: 22539306]
Salton M, Elkon R, Borodina T et al. Matrin 3 binds and stabilizes mRNA. PLoS One 2011 [PMID: 21858232]
Erazo A, Yee MB, Banfield BW et al. The alphaherpesvirus US3/ORF66 protein kinases direct phosphorylation of the nuclear matrix protein matrin 3. J Virol 2011 Jan [PMID: 20962082]
Schessl J, Zou Y, McGrath MJ et al. Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy. J Clin Invest 2008 Mar [PMID: 18274675]

Review for Matrin 3 Antibody (NB100-1761) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1761:
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Images Ratings Applications Species Date Details
Immunoprecipitation Matrin 3 NB100-1761
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reviewed by:
Jennifer Fox
 IP Human 09/12/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedHuman fibroblasts
SpeciesHuman
LotA1
CommentsRabbit IgG at 2ug:500ug lysate as negative control (Lane 2). 40ug of input as positive control (Lane 1)Cells lysed in 1X RIPA containing phosphatase and protease inhibitors.

Blocking

Blocking DetailsNA

Primary Anitbody

Dilution Ratio2ug:500ug lysate o/n 4C for IP

Details

Immunoprecipitation DetailsProtein A beads, 50ul slurry. 2H pre-clear, 2ug AB:500ug Lysate o/n 4C, 2H Beads/Lysate/AB 4C. 4 washes in 1X Ripa 4C
Elution Description1X LDS buffer, no DTT 10 minutes room temperature

Comments

CommentsRabbit IgG at 2ug:500ug lysate as negative control (Lane 2). 40ug of input as positive control (Lane 1)Cells lysed in 1X RIPA containing phosphatase and protease inhibitors.

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Immunoprecipitation Matrin 3 NB100-1761
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 Jennifer Fox
09/12/2014
Application: IP
Species: Human
read this review

Bioinformatics

Gene Symbol MATR3
Entrez
Uniprot