Mast Cell Protease-1/Mcpt1 Antibody [Unconjugated]

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Mast Cell Protease-1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease‑1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse Mast Cell Protease‑1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Mast Cell Protease-1/Mcpt1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
  Glu19-Leu246
  Accession # P11034
• Specificity: Detects mouse Mast Cell Protease‑1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Mcpt6, rmMcpt7, and rmMcpt11 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Mcpt1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mast Cell Protease-1/Mcpt1 Antibody [Unconjugated]

• AV080368
• Mast Cell Protease1
• Mast Cell Protease-1
• Mcp1
• Mcp-1
• MMPC-1

Background

Mast cell protease-1 (Mcpt1), also known as beta ‑chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMcpt1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N‑terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha ‑Chymase, Mcpt1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
2. Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
3. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
4. Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
5. Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
6. Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mast Cell Protease-1/Mcpt1 Antibody (AF5146)(2)

Publications using AF5146

• Kuo, N;Li, P;Cunha, JB;Chen, L;Shavit, JA;Omary, MB; The Histamine Pathway is a Target to Treat Hepatic Experimental Erythropoietic Protoporphyria Cellular and molecular gastroenterology and hepatology 2025-01-15 [PMID: 39824305] (Western Blot, Mouse)
• P Widmayer, P Pregitzer, H Breer Short-term high fat feeding induces inflammatory responses of tuft cells and mucosal barrier cells in the murine stomach Histology and histopathology, 2022-07-29;0(0):18503. 2022-07-29 [PMID: 35904321] (IHC, Mouse)

Bioinformatics

• Gene Symbol: Mcpt1
• Uniprot:
  • P11034()