MARK4 Antibody (OTI9B7) [DyLight 650]

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: OTI9B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

MARK4 Antibody (OTI9B7) [DyLight 650] Summary

• Immunogen: Recombinant protein fragment corresponding to amino acids 390-467 of human MARK4 (NP_113605) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MARK4 Antibody (OTI9B7) [DyLight 650]

• EC 2.7.11
• EC 2.7.11.1
• FLJ12177
• FLJ90097
• KIAA1860MARK4L
• MAP/microtubule affinity-regulating kinase 4
• MAP/microtubule affinity-regulating kinase like 1
• MAP/microtubule affinity-regulating kinase-like 1
• MARK4 serine/threonine protein kinase
• MARKL1L
• MARKL1MARK4S
• Nbla00650

Background

MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARK4