MARCO Antibody (PLK-1) - Azide Free

• Reactivity: Hu, Bv
• Applications: WB, Flow, Func, ICC/IF, IHC, IP
• Clone: PLK-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

MARCO Antibody (PLK-1) - Azide Free Summary

• Description: Novus Biologicals Mouse MARCO Antibody (PLK-1) - Azide Free (NBP3-11289) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human alveolar macrophages (recognizes domain V between residues 420 and 431)
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARCO
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: For Immunohistochemistry and Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of MARCO to be inactivated.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for MARCO Antibody (PLK-1) - Azide Free

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

The monoclonal antibody PLK-1 recognizes the macrophage receptor with collagenous structure (MARCO). The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although its expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens. The monoclonal antibody PLK-1 binds specifically to MARCO, and has been shown to partially block ligand binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCO