MARCH8 Antibody - BSA Free

Western Blot: MARCH8 Antibody [NBP1-76959] - HeLa cell lysate with MARCH8 antibody at (A) 0.5 ug/ml and (B) 1 ug/mL.

Immunocytochemistry/Immunofluorescence: MARCH8 Antibody [NBP1-76959] - HeLa cells with MARCH8 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MARCH8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MARCH8 Antibody - BSA Free (NBP1-76959) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8. The immunogen is located within the last 50 amino acids of MARCH8. Amino Acid Squence: CPETSKKNIFEKSPLTE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARCHF8
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/mL
  • Western Blot 0.5-1 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MARCH8 Antibody - BSA Free

• Cellular modulator of immune recognition
• c-MIR
• EC 6.3.2
• EC 6.3.2.-
• MARCH8
• MARCH-VIII
• MARCH-VIIIcellular modulator of immune recognition
• membrane-associated ring finger (C3HC4) 8
• Membrane-associated RING finger protein 8
• Membrane-associated RING-CH protein VIII
• MIR
• RING finger protein 178
• RNF178

Background

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARCHF8
• Uniprot:
  • NP_659458(Human)