MARCH8 Antibody

Images

 
Western Blot: MARCH8 Antibody [NBP1-76959] - HeLa cell lysate with MARCH8 antibody at (A) 0.5 ug/ml and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: MARCH8 Antibody [NBP1-76959] - HeLa cells with MARCH8 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MARCH8 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8. The immunogen is located within the last 50 amino acids of MARCH8.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARCH8
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARCH8 Antibody

  • Cellular modulator of immune recognition
  • c-MIR
  • EC 6.3.2
  • EC 6.3.2.-
  • MARCH8
  • MARCH-VIII
  • MARCH-VIIIcellular modulator of immune recognition
  • membrane-associated ring finger (C3HC4) 8
  • Membrane-associated RING finger protein 8
  • Membrane-associated RING-CH protein VIII
  • MIR
  • RING finger protein 178
  • RNF178

Background

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARCH8
Entrez
Uniprot