MAP4K5 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

MAP4K5 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human MAP4K5
  Lys395-Asn484 (Asn473Lys)
  Accession # Q9Y4K4
• Specificity: Detects human MAP4K5 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MAP4K5 Antibody [Alexa Fluor® 350]

• EC 2.7.11
• EC 2.7.11.1
• GCKR
• germinal center kinase-related
• KHS1
• KHSMEKKK 5
• Kinase homologous to SPS1/STE20
• MAP4K5
• MAPK/ERK kinase kinase kinase 5
• MAPKKKK5
• MEK kinase kinase 5
• mitogen-activated protein kinase kinase kinase kinase 5

Background

MAP4K5 (MAP Kinase Kinase Kinase Kinase 5; also known as KHS, MEKKK5, and GCKR) is a 94-96 kDa member of the STE (STErile 20 protein) subfamily, STE Ser/Thr protein kinase family, protein kinase superfamily of enzymes. It is ubiquitously expressed, and serves as an "intermediary" between GPCRs and cytosolic MAP kinases. It also interacts with Crk (CT10 Regulator of Kinase)-related adaptor molecules, facilitating the formation of signaling complexes around Tyr phosphorylated molecules. And finally, it represents an upstream component of a TNFR1 signaling pathway that requires activation of stress-activated protein kinases. Human MAP4K5 is 846 amino acids 9aa) in length. It contains a 258 aa kinase domain (aa 20-277) that is coupled to a 316 aa CNH (citron homology) domain (aa 506-826) which typically binds GTPases. There are two isoform variants. One shows a deletion of both Pro481 and Glu569, while another possesses a substitution of 16 aa for aa 569-846. Over aa 395-484, human MAP4K5 shares 83% aa sequence identity with mouse MAP4K5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.