MAP4K1 Antibody

Images

 
Western Blot: MAP4K1 Antibody [NB100-1045] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MAP4K1 Antibody Summary

Immunogen
Peptide with sequence DVVDPDIFNRDPR-C corresponding to N-Terminus according to NP_001036065.1, NP_009112.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAP4K1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 95-100 kDa band observed in Jurkat (predicted MW of 96 kDa band according to NP_009112).
IHC: Paraffin embedded Human Thymus.
Control
Jurkat Whole Cell Lysate
Publications
Read Publication using NB100-1045.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAP4K1 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Hematopoietic progenitor kinase
  • HPK1hematopoietic progenitor kinase 1
  • MAPK/ERK kinase kinase kinase 1
  • MEK kinase kinase 1
  • MEKKK 1
  • mitogen-activated protein kinase kinase kinase kinase 1

Background

MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAP4K1 Antibody (NB100-1045)(1)

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAP4K1
Entrez