MAP4K1 Antibody

Western Blot: MAP4K1 Antibody [NB100-1045] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MAP4K1 Antibody Summary

• Immunogen: Peptide with sequence DVVDPDIFNRDPR-C corresponding to N-Terminus according to NP_001036065.1, NP_009112.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAP4K1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 95-100 kDa band observed in Jurkat (predicted MW of 96 kDa band according to NP_009112).
  IHC: Paraffin embedded Human Thymus.
• Control:

  Jurkat Whole Cell Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAP4K1 Antibody

• EC 2.7.11
• EC 2.7.11.1
• Hematopoietic progenitor kinase
• HPK1hematopoietic progenitor kinase 1
• MAPK/ERK kinase kinase kinase 1
• MEK kinase kinase 1
• MEKKK 1
• mitogen-activated protein kinase kinase kinase kinase 1

Background

MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP4K1 Antibody (NB100-1045)(1)

Publication using NB100-1045

• Hu MC, Qiu WR, Wang X et al. Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. Genes Dev 1996-09-15 [PMID: 8824585]

Bioinformatics

• Gene Symbol: MAP4K1
• Entrez:
  • 11184(Human)